Zorbax Rx/Rx-Sil

Zorbax Rx/Rx Sil Guard Columns from Agilent Technologies

Choosing to use guard columns can help reduce operating expenses, by reducing the frequency of analytical column replacement. The guard column prevents damage caused by particulate matter and strongly adsorbed material. To maintain an adequate capacity for sample impurities, choose a guard column with an internal diameter similar to the column internal diameter.

Ideally, the packing of the guard column should be the same as the analytical column so that the chromatography of the analytical column is not altered. Guard columns contribute to the separation, so you should include a guard column inline during method development. Agilent UHPLC guards provide protection for high-efficiency Poroshell 120and ZORBAX RRHD and RRHT columns, without reducing performance.

ZORBAX Rx columns are made from pure porous silica microspheres and are optimized for high throughput analysis. Rx-C18 is recommended for alternate selectivity at low pH relative to Eclipse XDB-C18 and StableBond SB-C18. With low acidity and metal content, Rx-SIL is ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica. The Rx-SIL is also stronger than other silica types and is less acidic than the ZORBAX-SIL. Rx columns are available in 1.8, and 5 μm particle sizes for high speed, high resolution and analytical separations.